| RLS | R L Studebaker 
| Others |
| RLS | Rail Lock Slide
| Others |
| RLS | Rail Lock System
| Others |
| RLS | Rain and Light Sensor
| Others |
| RLS | Rainbow Lake Software
| Others |
| RLS | Ralph Lauren Sports
| Others |
| RLS | Rapid Locking System
| Others |
| RLS | Real Living with Slide (RV model)
| Others |
| RLS | Real-Life Server
| Others |
| RLS | Really Lazy Students
| Others |
| RLS | REBNY Listing Service
| Others |
| RLS | Reconfigurable Line System
| Others |
| RLS | Record Level Sharing
| Others |
| RLS | Record Level Subscriber
| Others |
| RLS | Recursive Least Means Squares
| Others |
| RLS | Recursive Least Squares
| Others |
| RLs | Regal Ls
| Others |
| RLS | Regional Library System
| Others |
| RLS | Registered Land Surveyor
| Others |
| RLS | Registration and Licensing System
| Others |
| RLS | Replica Location Service
| Others |
| RLS | Reserve Legal Support
| Others |
| RLS | Residential Listing Service
| Others |
| RLS | Resource Locator Service
| Others |
| RLS | Restless Leg Syndrome
| Others |
| RLS | Restless Legs Syndrome
| Others |
| RLS | Retirement Living Service
| Others |
| RLS | Retirement Living Services
| Others |
| RLS | Reweighed Least Squares
| Others |
| RLS | Rice Lake Speedway
| Others |
| RLS | Ricks Loyal Supporters
| Others |
| RLS | Rifle Length Shelf
| Others |
| RLS | River of Life Sanctuary
| Others |
| RLS | Road Load Simulator
| Others |
| RLS | Robert Lee Studebaker, Dallas Police Officer present at the Kennedy Assassination
| Others |
| RLS | Robert Louis Stevenson
| Others |
| RLS | Roccus Leneatus Saxatilis
| Others |
| RLS | Roland Lee Smart
| Others |
| RLS | Root Lateral Spread
| Others |
| RLS | Ross Lynch Source
| Others |
| RLS | Rotary And Linear Sensors
| Others |
| RLS | Route Listening Service
| Others |
| RLS | Row Level Security
| Others |
| RLS | Roxbury Latin School
| Others |
| RLs | Royale Ls
| Others |
| RLS | Rust Language Server
| Others |