| MLO | Main Line Operator 
| Others |
| MLO | Major Learning Objective
| Others |
| MLO | Major Learning Outcome
| Others |
| MLO | Malibu Locals Only
| Others |
| MLO | Mandriva Linux One
| Others |
| MLO | Mandriva Linux Online
| Others |
| MLO | Maria's Lash Out
| Others |
| MLO | Marine Life Observatory
| Others |
| MLO | Mason Lake Ocean
| Others |
| MLO | Material Liaison Office
| Others |
| MLO | Materiel Liaison Office
| Others |
| MLO | Mauna Loa Observatory
| Others |
| MLO | Maximum Loss Optimization
| Others |
| MLO | McCaig Law Office
| Others |
| MLO | McCAIG LAW OFFICES
| Others |
| MLO | Mclaren Orange
| Others |
| MLO | Medical Laboratory Observer
| Others |
| MLO | Mediolateral Oblique
| Others |
| MLO | Messianic Literature Outreach
| Others |
| MLO | Metal Lift Off
| Others |
| MLO | Metro Ligero Oeste
| Others |
| MLO | Microsoft Licensing Optimization
| Others |
| MLO | Mildew-resistance LOcus gene
| Others |
| MLO | Military Liaison Office
| Others |
| MLO | Mill Levy Override
| Others |
| MLO | Milos, Greece
| Others |
| MLO | Ministers Licensing and Ordination
| Others |
| MLO | Misanthropic Luciferian Order
| Others |
| MLO | Misce Looney Ous
| Others |
| MLO | Model Lighting Ordinance
| Others |
| MLO | Mollicute-Like Organism
| Others |
| MLO | Monklands Light Opera
| Others |
| MLO | Mortgage Loan Officer
| Others |
| MLO | Mortgage Loan Origination
| Others |
| MLO | Mortgage Loan Originator
| Others |
| MLO | Mosquito Larvicide Oil
| Others |
| MLO | Most Likely Outcome
| Others |
| MLO | Motor Licensing Officer
| Others |
| MLO | Mount Laguna Observatory
| Others |
| MLO | Multiple Level Observation
| Others |
| MLO | My Life Organized
| Others |
| MLO | My Line Overboard
| Others |
| MLO | The Mens Liberation Organization
| Others |